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rapid immuno precipitation assay buffer  (Boston BioProducts)


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    Structured Review

    Boston BioProducts rapid immuno precipitation assay buffer
    Rapid Immuno Precipitation Assay Buffer, supplied by Boston BioProducts, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rapid+immuno+precipitation+assay+buffer/pm31109340-130-24-29
    Average 86 stars, based on 1 article reviews
    rapid immuno precipitation assay buffer - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Immunoprecipitation:

    Article Title: Role of Hsp-70 in Triptolide-Mediated Cell Death of Neuroblastoma
    Article Snippet: .. Cell lysates were prepared by resuspending cells in Rapid Immuno Precipitation Assay Buffer (Boston BioProducts, Worcester, Massachusetts) with protease inhibitor (20 μL per mL of lysis buffer) for two hours on ice, and cleared by centrifugation for 10 minutes at 18000 rcf. .. Following supernatant collection, protein concentration was determined using a bicinchoninic acid protein assay (Pierce Biotechnology, Rockford, Illinois) with bovine serum albumin standards.

    Article Title: Role of Hsp-70 in triptolide-mediated cell death of neuroblastoma.
    Article Snippet: .. Cell lysates were prepared by resuspending cells in Rapid Immuno Precipitation Assay Buffer (Boston BioProducts, Worcester, MA) with protease inhibitor (20 mL per mL of lysis buffer) for 2 h on ice, and cleared by centrifugation for 10 min at 18,000 rcf. .. Following supernatant collection, protein concentration was determined using a bicinchoninic acid protein assay (Pierce Biotechnology, Rockford, IL) with bovine serum albumin standards.

    Article Title: Minnelide synergizes with conventional chemotherapy by targeting both cancer and associated stroma components in pancreatic cancer.
    Article Snippet: .. Cell lysates were prepared by removing the culture media, washing the cells with phosphate buffered saline and then adding Rapid Immuno Precipitation Assay Buffer (Boston BioProducts, MA) with protease (20 μL per milliliter of lysis buffer) and phosphatase (10 μL per milliliter of lysis buffer) inhibitors at 4 ◦C. ..

    Article Title: Triptolide-mediated cell death in neuroblastoma occurs by both apoptosis and autophagy pathways and results in inhibition of nuclear factor–kappa B activity
    Article Snippet: For the analysis of light-chain 3 (LC3), protease inhibitors E64-d (1 mg/mL in DMSO) and Pepstatin A (5 mg/mL in DMSO) (Sigma-Aldrich, St. Louis, MO) were added to the treatment medium at the time of triptolide dosing. .. Cell lysates were prepared by removing the culture medium and adding Rapid Immuno Precipitation Assay Buffer (Boston Bio-Products Inc, Worcester, MA) with protease (20 μL per milliliter of lysis buffer) and phosphatase (10 μL per milliliter of lysis buffer) inhibitors on ice. ..

    Article Title: Pre-clinical evaluation of Minnelide as a therapy for acute myeloid leukemia.
    Article Snippet: Primers were obtained from Integrated DNA Technologies Inc (IA) and are detailed in Additional file 3: Table S1. .. For western blotting, AML cells after treatment with indicated drugs and concentrations were washed with PBS once and cell lysates were prepared by adding Rapid Immuno Precipitation Assay Buffer (Boston Bioproducts, MA) with protease (Catalog Number: A32963, Thermo Scientific, MA) (20 μl/ml of lysis buffer) and phosphatase inhibitor (Catalog Number: 78420, Thermo Scientific, MA) in ice. .. The lysates thus obtained were quantified using Pierce bicinchoninic acid protein estimation kit (Catalog Number: 23250, Thermo Scientific, MA) and electrophoresis was performed on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels by loading 30–50 μg of protein.

    Article Title: Triptolide therapy for neuroblastoma decreases cell viability in vitro and inhibits tumor growth in vivo.
    Article Snippet: Background.. Heat shock protein (Hsp)-70 is overexpressed in several human malignancies, and its inhibition has been shown to kill cancer cells.. Our objectives were to assess the effectiveness of triptolide, an Hsp-70 inhibitor, in treating neuroblastoma in vitro and in vivo, and to measure the associated effects on Hsp-70 levels and apoptosis markers.

    Protease Inhibitor:

    Article Title: Role of Hsp-70 in Triptolide-Mediated Cell Death of Neuroblastoma
    Article Snippet: .. Cell lysates were prepared by resuspending cells in Rapid Immuno Precipitation Assay Buffer (Boston BioProducts, Worcester, Massachusetts) with protease inhibitor (20 μL per mL of lysis buffer) for two hours on ice, and cleared by centrifugation for 10 minutes at 18000 rcf. .. Following supernatant collection, protein concentration was determined using a bicinchoninic acid protein assay (Pierce Biotechnology, Rockford, Illinois) with bovine serum albumin standards.

    Article Title: Role of Hsp-70 in triptolide-mediated cell death of neuroblastoma.
    Article Snippet: .. Cell lysates were prepared by resuspending cells in Rapid Immuno Precipitation Assay Buffer (Boston BioProducts, Worcester, MA) with protease inhibitor (20 mL per mL of lysis buffer) for 2 h on ice, and cleared by centrifugation for 10 min at 18,000 rcf. .. Following supernatant collection, protein concentration was determined using a bicinchoninic acid protein assay (Pierce Biotechnology, Rockford, IL) with bovine serum albumin standards.

    Article Title: Triptolide therapy for neuroblastoma decreases cell viability in vitro and inhibits tumor growth in vivo.
    Article Snippet: Background.. Heat shock protein (Hsp)-70 is overexpressed in several human malignancies, and its inhibition has been shown to kill cancer cells.. Our objectives were to assess the effectiveness of triptolide, an Hsp-70 inhibitor, in treating neuroblastoma in vitro and in vivo, and to measure the associated effects on Hsp-70 levels and apoptosis markers.

    Lysis:

    Article Title: Role of Hsp-70 in Triptolide-Mediated Cell Death of Neuroblastoma
    Article Snippet: .. Cell lysates were prepared by resuspending cells in Rapid Immuno Precipitation Assay Buffer (Boston BioProducts, Worcester, Massachusetts) with protease inhibitor (20 μL per mL of lysis buffer) for two hours on ice, and cleared by centrifugation for 10 minutes at 18000 rcf. .. Following supernatant collection, protein concentration was determined using a bicinchoninic acid protein assay (Pierce Biotechnology, Rockford, Illinois) with bovine serum albumin standards.

    Article Title: Role of Hsp-70 in triptolide-mediated cell death of neuroblastoma.
    Article Snippet: .. Cell lysates were prepared by resuspending cells in Rapid Immuno Precipitation Assay Buffer (Boston BioProducts, Worcester, MA) with protease inhibitor (20 mL per mL of lysis buffer) for 2 h on ice, and cleared by centrifugation for 10 min at 18,000 rcf. .. Following supernatant collection, protein concentration was determined using a bicinchoninic acid protein assay (Pierce Biotechnology, Rockford, IL) with bovine serum albumin standards.

    Article Title: Minnelide synergizes with conventional chemotherapy by targeting both cancer and associated stroma components in pancreatic cancer.
    Article Snippet: .. Cell lysates were prepared by removing the culture media, washing the cells with phosphate buffered saline and then adding Rapid Immuno Precipitation Assay Buffer (Boston BioProducts, MA) with protease (20 μL per milliliter of lysis buffer) and phosphatase (10 μL per milliliter of lysis buffer) inhibitors at 4 ◦C. ..

    Article Title: Triptolide-mediated cell death in neuroblastoma occurs by both apoptosis and autophagy pathways and results in inhibition of nuclear factor–kappa B activity
    Article Snippet: For the analysis of light-chain 3 (LC3), protease inhibitors E64-d (1 mg/mL in DMSO) and Pepstatin A (5 mg/mL in DMSO) (Sigma-Aldrich, St. Louis, MO) were added to the treatment medium at the time of triptolide dosing. .. Cell lysates were prepared by removing the culture medium and adding Rapid Immuno Precipitation Assay Buffer (Boston Bio-Products Inc, Worcester, MA) with protease (20 μL per milliliter of lysis buffer) and phosphatase (10 μL per milliliter of lysis buffer) inhibitors on ice. ..

    Article Title: Pre-clinical evaluation of Minnelide as a therapy for acute myeloid leukemia.
    Article Snippet: Primers were obtained from Integrated DNA Technologies Inc (IA) and are detailed in Additional file 3: Table S1. .. For western blotting, AML cells after treatment with indicated drugs and concentrations were washed with PBS once and cell lysates were prepared by adding Rapid Immuno Precipitation Assay Buffer (Boston Bioproducts, MA) with protease (Catalog Number: A32963, Thermo Scientific, MA) (20 μl/ml of lysis buffer) and phosphatase inhibitor (Catalog Number: 78420, Thermo Scientific, MA) in ice. .. The lysates thus obtained were quantified using Pierce bicinchoninic acid protein estimation kit (Catalog Number: 23250, Thermo Scientific, MA) and electrophoresis was performed on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels by loading 30–50 μg of protein.

    Article Title: Triptolide therapy for neuroblastoma decreases cell viability in vitro and inhibits tumor growth in vivo.
    Article Snippet: Background.. Heat shock protein (Hsp)-70 is overexpressed in several human malignancies, and its inhibition has been shown to kill cancer cells.. Our objectives were to assess the effectiveness of triptolide, an Hsp-70 inhibitor, in treating neuroblastoma in vitro and in vivo, and to measure the associated effects on Hsp-70 levels and apoptosis markers.

    Centrifugation:

    Article Title: Role of Hsp-70 in Triptolide-Mediated Cell Death of Neuroblastoma
    Article Snippet: .. Cell lysates were prepared by resuspending cells in Rapid Immuno Precipitation Assay Buffer (Boston BioProducts, Worcester, Massachusetts) with protease inhibitor (20 μL per mL of lysis buffer) for two hours on ice, and cleared by centrifugation for 10 minutes at 18000 rcf. .. Following supernatant collection, protein concentration was determined using a bicinchoninic acid protein assay (Pierce Biotechnology, Rockford, Illinois) with bovine serum albumin standards.

    Article Title: Role of Hsp-70 in triptolide-mediated cell death of neuroblastoma.
    Article Snippet: .. Cell lysates were prepared by resuspending cells in Rapid Immuno Precipitation Assay Buffer (Boston BioProducts, Worcester, MA) with protease inhibitor (20 mL per mL of lysis buffer) for 2 h on ice, and cleared by centrifugation for 10 min at 18,000 rcf. .. Following supernatant collection, protein concentration was determined using a bicinchoninic acid protein assay (Pierce Biotechnology, Rockford, IL) with bovine serum albumin standards.

    Article Title: Triptolide therapy for neuroblastoma decreases cell viability in vitro and inhibits tumor growth in vivo.
    Article Snippet: Background.. Heat shock protein (Hsp)-70 is overexpressed in several human malignancies, and its inhibition has been shown to kill cancer cells.. Our objectives were to assess the effectiveness of triptolide, an Hsp-70 inhibitor, in treating neuroblastoma in vitro and in vivo, and to measure the associated effects on Hsp-70 levels and apoptosis markers.

    Saline:

    Article Title: Minnelide synergizes with conventional chemotherapy by targeting both cancer and associated stroma components in pancreatic cancer.
    Article Snippet: .. Cell lysates were prepared by removing the culture media, washing the cells with phosphate buffered saline and then adding Rapid Immuno Precipitation Assay Buffer (Boston BioProducts, MA) with protease (20 μL per milliliter of lysis buffer) and phosphatase (10 μL per milliliter of lysis buffer) inhibitors at 4 ◦C. ..

    Article Title: Minnelide Overcomes Oxaliplatin Resistance by Downregulating the DNA Repair Pathway in Pancreatic Cancer.
    Article Snippet: .. Cell lysates were prepared by removing the culture media, washing the cells with phosphate-buffered saline, and then adding Rapid Immuno Precipitation Assay Buffer (Boston BioProducts, MA) with protease (20 μL per milliliter of lysis buffer) and phosphatase (10 μL per milliliter of lysis buffer) inhibitors at 4 °C. ..

    Western Blot:

    Article Title: Pre-clinical evaluation of Minnelide as a therapy for acute myeloid leukemia.
    Article Snippet: Primers were obtained from Integrated DNA Technologies Inc (IA) and are detailed in Additional file 3: Table S1. .. For western blotting, AML cells after treatment with indicated drugs and concentrations were washed with PBS once and cell lysates were prepared by adding Rapid Immuno Precipitation Assay Buffer (Boston Bioproducts, MA) with protease (Catalog Number: A32963, Thermo Scientific, MA) (20 μl/ml of lysis buffer) and phosphatase inhibitor (Catalog Number: 78420, Thermo Scientific, MA) in ice. .. The lysates thus obtained were quantified using Pierce bicinchoninic acid protein estimation kit (Catalog Number: 23250, Thermo Scientific, MA) and electrophoresis was performed on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels by loading 30–50 μg of protein.



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